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「シデリティス」は｢ギリシャ 山のお茶｣(Greek Mountain Tea)と呼ばれ、
数千年も前からギリシャ周辺の地域、
標高約1000ｍの岩場で自生するシソ科のハーブです。
「薬理学の父」ディオスコリデスも治療に用いてきました。
今ではドイツ・アメリカをはじめ多くの国で飲まれています。
＊これらの論文は研究報告に基づくものです。(使用言語：英語)
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North American Menopause Society, 2011 Aug;18(8):915-22. |
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Protective effect of Sideritis euboea extract on bone mineral density and strength of ovariectomized rats. |
Dontas IA, Lelovas PP, Kourkoulis SK, Aligiannis N, Paliogianni A, Mitakou S,
Galanos A, Kassi E, Mitousoudis A, Xanthos TT, Papaioannou N, Lyritis GP. |
SourceLaboratory for Research of the Musculoskeletal System,
School of Medicine, University of Athens, Greece. |
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Abstract
OBJECTIVE: The aim of this study was to investigate the potential protective effect of Sideritis euboea extract (SID), commonly consumed as "mountain tea," on bone mineral density (BMD) and the strength of the ovariectomized (OVX) rat model of osteoporosis.
METHODS: Thirty-two 10-month-old Wistar rats were separated into controls (sham operated), OVX, and OVX plus SID in their drinking water (dose, 330 mg/kg body weight per day), starting immediately after OVX for 6 months. Tibial BMD at baseline and at 3 and 6 months post-OVX, three-point-bending of the femur, and body and uterine weight at the study end were examined.
RESULTS: BMD percentage change from baseline of the whole tibia was similar in control and OVX + SID rats at 3 months (-3.02% vs -4.67%, P = not significant), revealing a strong osteoprotective effect. At 6 months, the corresponding changes were -6.02% versus -14.37%, P < 0.05, indicating a greater bone loss in treated rats, albeit significantly less than the OVX change (-20.46%; OVX vs OVX + SID, P < 0.05). The proximal (metaphyseal) tibial BMD percentage change from baseline to 3 and 6 months between the OVX and OVX + SID groups (-26.47% vs -15.57% and -31.22% vs -16.57%, respectively) was statistically significant, demonstrating that SID preserved the proximal tibial BMD of the OVX + SID group significantly. Three-point-bending showed a significant increase in the treated compared with the OVX groups. Body and uterine weights were similar in the OVX and treated groups.
CONCLUSIONS: SID significantly protected tibial bone loss and improved femoral biomechanical strength in OVX + SID rats compared with OVX rats.
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Extracts of Sideritis scardica as inhibitors of monoamine transporters:
A pharmacological mechanism for efficacy in mood disorders and attention-deficit hyperactivity disorders (ADHD) |
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Rainer Knörle |
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IBAM GbR Dr. Rainer Knörle & Dr. Peter Schnierle, Ferdinand-Porsche-Strasse 5, D-79211 Denzlingen, Germany |
Abstract
Aim of the study: Sideritis species are traditionally used within the Mediterranean area for the cure of cold cough and for the treatment of gastrointestinal disorders. The aim of this study was to investigate the effect of Sideritis scardica extracts on the monoamine transporter and to derive possible medicinal application from the pharmacological profile of the extracts.
Methods: We have studied the effect of various Sideritis scardica extracts on serotonin, noradrenaline and dopamine uptake into rat brain synaptosomes and serotonin uptake into human JAR cells.
Results: All extracts inhibited the uptake of all three monoamines into rat brain synaptosomes by their respective transporters, the alcoholic extracts being more effective than the water extract. EC50 were in the range of 30-40 μg/ml. Inhibition of the human serotonin transporter by the menthol extract was even more effective (EC50: 1.4μg/ml). Combining Sideritis ethanol extract and fluvoxamine resulted in a leftward shift of the fluvoxamine concentration –response curve.
Conclusions: The pharmacological profile of Sideritis scardica extracts suggests their use in the phytochemical therapy of mental disorders associated with a malfunctioning monoaminergic neurotransmission, like major depression or the attention-deficit hyperactivity disorder.
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J. Agric. Food Chem., 2004, 52 (23), pp 6956–6961 DOI: 10.1021/jf0400765 |
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Greek Plant Extracts Exhibit Selective Estrogen Receptor Modulator (SERM)-like Properties |
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Eva Kassi,† Zoi Papoutsi,† Nikolaos Fokialakis,‡ Ioanna Messari,§ Sophia Mitakou,‡ and Paraskevi Moutsatsou*† |
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Department of Biological Chemistry, Medical School, University of Athens, 115 27 Athens, Greece, Department of Pharmacognosy, Department of Pharmacy, University of Athens, 115 27 Athens, Greece, and Department of Experimental Pharmacology, Medical School, University of Athens, 115 27 Athens, Greece |
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Abstract
To prevent bone loss that occurs with increasing age, nutritional and pharmacological factors are needed. Traditional therapeutic agents (selective estrogen receptor modulators or SERMs, biphosphonates, calcitonin) may have serious side effects or contraindications. In an attempt to find food components potentially acting as SERMs, we submitted four plant aqueous extracts derived from Greek flora (Sideritis euboea, Sideritis clandestina, Marticaria chamomilla, and Pimpinella anisum) in a series of in vitro biological assays reflective of SERM profile. We examined their ability (a) to stimulate the differentiation and mineralization of osteoblastic cell culture by histochemical staining for alkaline phosphatase and Alizarin Red-S staining, (b) to induce, like antiestrogens, the insulin growth factor binding protein 3 (IGFBP3) in MCF-7 breast cancer cells, and (c) to proliferate cervical adenocarcinoma (HeLa) cells by use of MTT assay. Our data reveal that all the plant extracts studied at a concentration range 10−100 μg/mL stimulate osteoblastic cell differentiation and exhibit antiestrogenic effect on breast cancer cells without proliferative effects on cervical adenocarcinoma cells. The presence of estradiol inhibited the antiestrogenic effect induced by the extracts on MCF-7 cells, suggesting an estrogen receptor-related mechanism. In conclusion, the aqueous extracts derived from Sideritis euboea, Sideritis clandestina, Marticaria chamomilla, and Pimpinella anisum may form the basis to design “functional foods” for the prevention of osteoporosis.
Keywords: Sideritis; Anisum; Chamomilla; selective estrogen receptor modulators; plant extracts
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Phytotherapy Research Volume 22, Issue 4, pages 450–454, April 2008
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Antioxidant and antiinflammatory activities of Sideritis perfoliata subsp. perfoliata (Lamiaceae) |
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Maria-Thiresia Charami1, Diamanto Lazari1,*, Anastasia Karioti3, Helen Skaltsa3, Dimitra Hadjipavlou-Litina2, Christos Souleles1 |
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1Laboratory of Pharmacognosy, School of Pharmacy, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece
2Department of Pharmaceutical Chemistry, School of Pharmacy, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece
3Department of Pharmacognosy and Chemistry of Natural Products, Schoo |
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Abstract
Sideritis perfoliata L. subsp. perfoliata is a plant widely used in folk medicine in Greece since antiquity because of its antiinflammatory, antirheumatic, antiulcer, digestive and vasoprotective properties.
Phytochemical investigations of the polar extracts afforded four flavonoid glycosides, four phenylpropanoic glycosides, caffeic acid and one iridoid, ajugoside. Reactive oxygen species (ROS) are implicated in the aetiology of several inflammatory processes. In the present study polar fractions and isolated compounds from S. perfoliata subsp. perfoliata were evaluated for their antioxidant activity using DPPH spectrophotometric and TBA lipid peroxidation assays, as well as for their antiinflammatory activity using the soybean lipoxygenase bioassay.
All extracts and isolated compounds showed significant antioxidant and inhibitory activity against soybean lipoxygenase. These findings give support to the ethnopharmacological use of the plant in the treatment of several inflammatory ailments.
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JOURNAL OF MEDICINAL FOOD J Med Food 14 (9) 2011, 1060–1064
DOI: 10.1089/jmf.2010.0187
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Differential Antioxidant Effects of Consuming Tea from Sideritis clandestina subsp. peloponnesiaca on Cerebral Regions of Adult Mice |
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Zacharoula I. Linardaki,1 Catherine G. Vasilopoulou,1 Caterina Constantinou,1,* Gregoris Iatrou,2 Fotini N. Lamari,3 and Marigoula Margarity1 |
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1Laboratory of Human and Animal Physiology, Department of Biology; 2Division of Plant Biology, Department of Biology; 3Laboratory of Pharmacognosy & Chemistry of Natural Products, Department of Pharmacy; University of Patras, Patras, Greece. |
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Abstract
Oxidative stress is involved in the pathophysiology of neurodegenerative diseases and aging. Many species of the genus Sideritis (mountain tea) are widely consumed in the Mediterranean region as herbal tea. This study evaluated the effect of supplementation of mice with herbal tea from Sideritis clandestina subsp. peloponnesiaca on the antioxidant status of different brain regions. To select the most bioactive herbal tea, the polyphenolic content (Folin–Ciocalteu method) and the antioxidant properties (ferric reducing antioxidant power [FRAP] and 2,2-diphenyl-1-picrylhydrazyl assays) of several taxa and different populations of the S. clandestina infusions were measured in vitro. Male adult mice had ad libitum access to water (control) or the herbal tea (4% w/v) for 6 weeks. At the end of the treatment period we assessed the total antioxidant power (FRAP assay) and the levels of malondialdehyde (indicator of lipid peroxidation) and reduced glutathione in the cerebral cortex, cerebellum, and midbrain. These biochemical measures have also been determined in liver samples used as a comparative reference peripheral tissue. Consumption of 4% herbal tea increased the total antioxidant power of the midbrain by 72% (P < .05); a significant (P < .05) decrease in malondialdehyde levels and increase in reduced glutathione content of the cerebellum (78% and 27%, espectively) and midbrain (59% and 32%, respectively) were also observed. These findings indicate that mountain tea consumption enhances the antioxidant defense of the adult rodent brain in a region-specific manner.
KEY WORDS: antioxidant capacity brain reduced glutathione lipid peroxidation mountain tea Sideritis |
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Natural Product Communications Vol. 6 (11) 2011 1689 - 1696 |
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Effects of Sideritis euboea (Lamiaceae) Aqueous Extract on IL-6, OPG and RANKL Secretion by Osteoblasts |
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Eva Kassia, Anna Paliogiannib, Ismene Dontasc, Nektarios Aligiannisb, Maria Halabalakib, Zoi Papoutsia, Alexios-Leandros Skaltsounisb,* and Paraskevi Moutsatsoua,* |
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aDepartment of Biological Chemistry, Medical School, University of Athens, 75 M. Asias, Goudi, 11527 Athens, Greece
bDivision of Pharmacognosy, Department of Pharmacy, University of Athens, Panepistimiopolis Zografou, 15771 Athens, Greece
cLaboratory for the Research of the Musculoskeletal System, Medical School, University of Athens, Athens, Greece |
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Abstract
The water extract obtained from the aerial parts of Sideritis euboea (Lamiaceae), which is known in Greece as ‘mountain tea’, was investigated by determining the in vitro effect of this extract on the IL-6, OPG and RANKL secretion by osteoblasts, three important molecules in osteoblast-osteoclast interplay. The results showed that this extract reduced significantly the secretion of IL-6 by KS-483 osteoblasts, while it also suppressed RANKL secretion, with both effects being dose-dependent and more potent at the higher concentrations tested (50, 100 μg/mL). We further determined the chemical profile of the extract by applying an analytical U-HPLC-DAD-ESI-MS/MS method using the high resolution hybrid LTQ-Orbitrap Discovery spectrometer. An ESI source in negative mode was employed. The analysis indicated that the water extract of S. euboea is rich in flavonoid glycosides, and phenylpropanoid glycosides.
Keywords: Sideritis, Lamiaceae, IL-6, OPG, RANKL, osteoblasts. |
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